JNK antibody (70R-12015)
Rabbit polyclonal JNK antibody
Overview
Overview
| Synonyms | Polyclonal JNK antibody, anti-JNK antibody, JAK1A antibody, Stress activated protein kinase antibody, JNK1A2 antibody, SARP antibody, JNK1 antibody, JNK antibody, JAK 1A antibody, JNK1 antibody, JNK 1 antibody |
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| Cross Reactivity | Human, Mouse, Rat |
| Applications | IHC, IP, WB |
| Immunogen | JNK antibody was raised in Rabbit using synthetic peptide surrounding amino acid 412 of human JNK-1 as the immunogen. |
| Assay Information | JNK Blocking Peptide, catalog no. 33R-10890, is also available for use as a blocking control in assays to test for specificity of this JNK antibody |
Images
Western Blot analysis using JNK antibody (70R-12015)
Western blot analysis of JNK expression in Jurkat cell lysates (Lane 1 & 2).
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | JNK antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | IP: 10-20 ug/ml, IHC: 10-20 ug/ml, WB: 0.5-4 ug/ml |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines, UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1, which in turn activates SAPK. JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71. |
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