Karyopherin alpha 2 antibody (70R-12987)

Affinity purified Rabbit polyclonal Karyopherin alpha 2 antibody

Synonyms Polyclonal Karyopherin alpha 2 antibody, Anti-Karyopherin alpha 2 antibody, QIP2 antibody, Karyopherin alpha -2, RAG cohort 1 antibody, IPOA1 antibody, pendulin antibody, KPNA2 antibody, SRP1alpha antibody, importin-alpha-P1 antibody, karyopherin subunit alpha-2 antibody, RAG cohort protein 1 antibody, Karyopherin alpha 2, Karyopherin alpha 2, Karyopherin alpha 2 antibody, SRP1-alpha antibody, Karyopherin alpha -2 antibody, RCH1 antibody, importin alpha 1 antibody, importin subunit alpha-2 antibody
Cross Reactivity Human
Applications ICC, IF, WB
Immunogen Karyopherin alpha 2 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 468 and 529 of karyopherin alpha 2

Images

Immunofluorescent staining using Karyopherin alpha 2 antibody (70R-12987)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using karyopherin alpha 2 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Karyopherin alpha 2 antibody was purified by antigen-affinity chromatography
Molecular Weight 58 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.71 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Karyopherin alpha 2 antibody (70R-12987)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using karyopherin alpha 2 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using Karyopherin alpha 2 antibody (70R-12987)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3; B:Hep G2; C:MOLT4) using a 10 % SDS PAGE gel and Karyopherin alpha 2 antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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