KHS antibody (70R-13268)

Affinity purified Rabbit polyclonal KHS antibody

Synonyms Polyclonal KHS antibody, Anti-KHS antibody, MEK kinase kinase 5 antibody, KHS antibody, MAPK/ERK kinase kinase kinase 5 antibody, MAPKKKK5 antibody, MEKKK 5 antibody, KHS1 antibody, GCKR antibody, MAP4K5 antibody, germinal center kinase-related antibody, kinase homologous to SPS1/STE20 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB

Images

Immunofluorescent staining using KHS antibody (70R-13268)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using KHS antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification KHS antibody was purified by antigen-affinity chromatography
Molecular Weight 95 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC-P: 1:100-1:500, WB: 1:1000-1:10000, IF:1:100-1:200, ICC:1:100-1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using KHS antibody (70R-13268)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A431, using KHS antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using KHS antibody (70R-13268)</font></div>     | Immunohistochemical staining of paraffin-embedded SAS xenograft using KHS antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using KHS antibody (70R-13268)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Molt-4) using a 12 % SDS PAGE gel and KHS antibody at a dilution of 1:5000

Availability: In stock

Price: €395.09
Size: 100 ul
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