KI67 antibody (20R-KR001)
Rabbit polyclonal KI67 antibody
Overview
Overview
| Synonyms | Polyclonal KI67 antibody, Anti-KI67 antibody, KI-67, KI67, KI-67 antibody, KI 67 antibody, KI 67 |
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| Specificity | Human |
| Cross Reactivity | Rat |
| Applications | IHC-F, IHC-P |
| Immunogen | KI67 antibody was raised in rabbit using a synthetic peptide derived from human Ki-67 Protein as the immunogen. |
Images
Immunohistochemical staining using KI67 antibody (20R-KR001)
Formalin-fixed, paraffin-embedded human tonsil stained with Ki67 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.
Specifications
| Host | Rabbit |
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| Method of Purification | KI67 antibody was purified by Protein A chromatography. |
| Form & Buffer | Supplied as a liquid with 0.2% BSA and 15mM NaN3. |
| Concentration | ~1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | IHC-F: 10-20 ug/ml, IHC-P: 10-20 ug/ml |
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Storage & Safety
| Storage | Store at 4 deg C. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling. |
General Information
| Biological Significance | Ki-67 is a nuclear protein, which is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. |
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