KLF4 protein (His CaM tag) (80R-1899)
Purified recombinant KLF4 protein
Overview
Overview
| Synonyms | KLF4, GKLF protein, KLF 4 protein, KLF-4 protein, KLF-4, KLF 4, EZF protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of KLF4 protein (His CaM tag) (80R-1899)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 11-395 amino acids: MAHHHHHHMA DQLTEEQIAE FKEAFSLFDK DGDGTITTKE LGTVMRSLGQ NPTEAELQDM INEVDADGNG TIDFPEFLTM MARKMKDTDS EEEIREAFRV FDKDGNGYIS AAELRHVMTN LGEKLTDEEV DEMIREADID GDGQVNYEEF VQMMTAKGSM AVSDALLPSF STFASGPAGR EKTLRQAGAP NNRWREELSH MKRLPPVLPG RPYDLAAATV ATDLESGGAG AACGGSNLAP LPRRETEEFN DLLDLDFILS NSLTHPPESV AATVSSSASA SSSSSPSSSG PASAPSTCSF TYPIRAGNDP GVAPGGTGGG LLYGRESAPP PTAPFNLADI NDVSPSGGFV AELLRPELDP VYIPPQQPQP PGGGLMGKFV LKASLSAPGS EYGSPSVISV SKGSPDGSHP VVVAPYNGGP PRTCPKIKQE AVSSCTHLGA GPPLSNGHRP AAHDFPLGRQ LPSRTTPTLG LEEVLSSRDC HPALPLPPGF HPHPGPNYPS FLPDQMQPQV PPLHYQELMP PGSCMPEEPK PKRGRRSWPR KRTAT |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 80% pure |
| Molecular Weight | 58.1 kDa (545aa) |
| Tag/Conjugate | His-Cam tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1M NaCl. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | KLF4 is a transcription factor which acts as both an activator and repressor. This protein is expressed principally in erythroid tissues and found predominantly in the gut. KLF4 is involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Recombinant human KLF4 protein, fused to His-CaM-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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