Lamin B1 antibody (70R-12721)

Affinity purified Rabbit polyclonal Lamin B1 antibody

Synonyms Polyclonal Lamin B1 antibody, Anti-Lamin B1 antibody, Lamin B-1 antibody, Lamin B1, Lamin B-1, MGC111419 antibody, LMNB1 antibody, LMN antibody, LMN2 antibody, ADLD antibody, lamin B1 antibody, Lamin B 1, Lamin B 1 antibody, LMNB antibody, lamin-B1 antibody
Cross Reactivity Human, Mouse
Applications IHC-P, WB
Immunogen Lamin B1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 289 and 574 of Lamin B1

Images

Western blot analysis using Lamin B1 antibody (70R-12721)

Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 7.5 % SDS PAGE gel and Lamin B1 antibody at a dilution of 1:5000

Specifications

Host Rabbit
Isotype IgG
Method of Purification Lamin B1 antibody was purified by antigen-affinity chromatography
Molecular Weight 66 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC-P: 1:100-1:500, WB: 1:1000-1:10000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using Lamin B1 antibody (70R-12721)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 7.5 % SDS PAGE gel and Lamin B1 antibody at a dilution of 1:5000
  • <div><font face=Calibri size=2>Immunohistochemical staining using Lamin B1 antibody (70R-12721)</font></div>     | Immunohistochemical staining of paraffin-embedded Colon ca using Lamin B1 antibody at a dilution of 1:250
  • <div><font face=Calibri size=2>Western blot analysis using Lamin B1 antibody (70R-12721)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:A431; B:H1299; C:HeLa S3) using a 7.5 % SDS PAGE gel and Lamin B1 antibody at a dilution of 1:5000

Availability: In stock

Price: €323.11
Size: 100 ul
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