Immunohistochemical staining using Lamin B2 antibody (70R-13318)
Immunohistochemical staining of paraffin-embedded Cal27 Xenograft using Lamin B2 antibody at a dilution of 1:100
Lamin B2 antibody
Immunohistochemical staining using Lamin B2 antibody (70R-13318)
Immunohistochemical staining of paraffin-embedded Cal27 Xenograft using Lamin B2 antibody at a dilution of 1:100
Western blot analysis using Lamin B2 antibody (70R-13318)
Western blot analysis of 30 ug of whole cell lysate (A: H1299; B: Hela; C: Hep G2) using a 7.5 % SDS PAGE gel and Lamin B2 antibody at a dilution of 1:1000
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Lamin B2 antibody was purified by antigen-affinity chromatography
Molecular Weight
68 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene.