LDH antibody (20C-CR6062GP)
Goat polyclonal LDH antibody
Overview
Overview
| Synonyms | Polyclonal LDH antibody, Anti-LDH antibody, L-lactate dehydrogenase A chain antibody, LDH-A antibody, LDH-M antibody, LDH muscle subunit antibody |
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| Specificity | Rabbit LDH |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IP, WB |
| Immunogen | LDH antibody was raised in goat using full length lactate dehydrogenase protein isolated from rabbit muscle as the immunogen. |
Images
Immunofluorescent staining of LDH antibody (20C-CR6062GP)
LDH antibody (biotin conj ugated) was used to stain HeLa cells by immunofluorescence. HeLa cells were plated in 12 well plates, fixed for 5 min in 1:1 MeTOH:Acetone, blocked for 15 min and stained 1 hr with at 1:200 in blocking buffer. Plate was washed 3X in PBS, and primary biotin conj ugate was detected by DyLight 488 conj ugated Streptavidin 1:10000 for 30 min. Well was washed 3X in PBS. Image was taken using EVOS fl All Digital Inverted Fluorescence Microscope by AMG (Advanced Microscopy Group).
Specifications
| Host | Goat |
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| Method of Purification | LDH antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis. |
| Form & Buffer | IgG fraction supplied as a liquid in PBS, pH 7.4, with 0.01% NaN3 |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:2,000-1:10,000, IP: 1:100, WB: 1:1,000-1:5,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H)-in the heart, LDH-2 (3H1M)-in the reticuloendothelial system, LDH-3 (2H2M)-in the lungs, LDH-4 (1H3M)-in the kidneys and LDH-5 (4M)-in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH |
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