LDHA protein (His tag) (80R-1263)

Purified recombinant Human LDHA protein

Synonyms L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein protein, Lactate dehydrogenase M protein, LDH muscle subunit protein, A chain protein, Lactate dehydrogenase 1 protein, LDH1 protein, LDHM protein, PIG19 protein, Lactate dehydrogenase A4 protein, M LDH protein, Renal carcinoma protein NY-REN-59., l7R2 protein, Proliferation-inducing gene 19 protein, GSD11 protein, L lactate dehydrogenase A chain protein, LDH muscle subunit protein, Lactate dehydrogenase A protein, OTTMUSP00000017774 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH 7.5 at 37 DEG C and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin. 2. Equilibrate to 37 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug, 0.5ug) in assay buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.

Images

Coomassie Blue stained SDS-PAGE of LDHA protein (His tag) (80R-1263)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-332 amino acids: MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 38.8 kDa (352aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol and 0.1M NaCl.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 20 units/mg. in which one unit will convert 1.0 uM of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37 DEG C.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance LDHA, also known as L-lactate dehydrogenase A chain, is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. Recombinant LDHA protein was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of LDHA protein (His tag) (80R-1263) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €206.99
Size: 100 ug
OR
Shipping
View Our Distributors