| Product Use |
Polyclonal LGR5 antibody specific for Human LGR5 for use in Western Blot.
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| Biological Significance |
LGR5 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), LGR5 associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
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| Expression |
Highly expressed in neural cells and is overexpressed in cancers of the ovary, colon and liver. In premalignant cancer expression is not restricted to the cript base. LGR5 is an integral membrane protein expressed in skeletal muscle, placenta, spinal cord, and various region of brain. LGR5 is also expressed at the base of crypts in colonic and small mucosa stem cells. |
| Implications in Disease |
LGR5 +ve stem cells were identified to fuel stem cell activity in murine intestinal adenomas via erroneous activation of the pro-cell cycle Wnt signalling pathway as a result of successive mutations, such as formation of adenoma via Adenomatous polyposis coli (APC) mutation. Loss of LGR5 actually increased tumourigenicity and invasion whereas overexpression results a reduction in tumourigenicity and clonogenicity. This implies that LGR5 is not an oncogene but a tumor suppressor gene, and that its main role is delimiting stem cell expansion in their respective niches. Varying expression profile of LGR5 was also observed in different stages of gastrointestinal cancers, which suggests that the histoanatomical distribution of LGR5+ve stem cells determine how the cancer advances. |
| Sequence Information |
LGR5 is composed of 907 amino acids with a molecular weight of 99998 Da. There is a Leucine-rich repeat-containing G-protein coupled receptor 5 chain at amino acids 22 – 907 and a signal peptide at positions 1 – 21. |
| Post-Translational Modifications |
LGR5 undergoes glycosylation2 at Asn63, Asn77, Asn208, Asn500 and Asn792. |
| Database Links |
Entrez Gene: 8549 Human, Omim: 606667 Human, SwissProt: O75473 Human, Unigene: 658889 Human |