LIM kinase 2 antibody (70R-13237)

Affinity purified Rabbit polyclonal LIM kinase 2 antibody

Synonyms Polyclonal LIM kinase 2 antibody, Anti-LIM kinase 2 antibody, LIM kinase 2, LIM kinase 2, LIM kinase -2, LIMK2 antibody, LIM domain kinase 2 antibody, LIM kinase -2 antibody, LIM kinase 2 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB

Images

Immunofluorescent staining using LIM kinase 2 antibody (70R-13237)

Immunofluorescence analysis of paraformaldehyde-fixed A549, using LIM kinase 2 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification LIM kinase 2 antibody was purified by antigen-affinity chromatography
Molecular Weight 78 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using LIM kinase 2 antibody (70R-13237)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A549, using LIM kinase 2 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using LIM kinase 2 antibody (70R-13237)</font></div>     | Immunohistochemical staining of paraffin-embedded Breast ca using LIM kinase 2 antibody at a dilution of 1:250
  • <div><font face=Calibri size=2>Western blot analysis using LIM kinase 2 antibody (70R-13237)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 7.5 % SDS PAGE gel and LIM kinase 2 antibody at a dilution of 1:2000

Availability: In stock

Price: €323.11
Size: 100 ul
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