LSM4 protein (His tag) (80R-1619)
Purified recombinant Human LSM4 protein
|Synonyms||U6 snRNA-associated Sm-like protein LSm4 protein, LSM 4, YER112W, LSM 4 protein, LSM-4, LSM-4 protein, LSM4|
Coomassie Blue stained SDS-PAGE of LSM4 protein (His tag) (80R-1619)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-139 amino acids: MGSSHHHHHH SSGLVPRGSH MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ|
|Grade & Purity||> 90% pure|
|Molecular Weight||17.5 kDa (159aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||LSM4, also called U6 snRNA-associated Sm-like protein or glycine-rich protein (GRP), forms donut-shaped heptameric complexes that are involved in various steps of RNA metabolism. It facilitates RNA protein interactions and structural changes that are required during ribosomal subunit assembly. It binds specifically to the 3'-terminal U-tract of U6 snRNA. Recombinant human LSM4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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