LYPLA1 protein (His tag) (80R-1482)

Purified recombinant mouse LYPLA1 protein

Synonyms LYPLA 1, Acyl-protein thioesterase 1 protein, LPL-I protein, LYPLA-1, LYPLA 1 protein, Pla1a protein, APT-1 protein, lysophospholipase 1 protein, APT1, LYPLA-1 protein, LYPLA1, LysoPLA I protein
Species Mouse
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of LYPLA1 protein (His tag) (80R-1482)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-230 amino acids: MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 26.8 kDa (250aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 0.1M NaCl and 1mM DTT.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity. Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

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Images

  • Coomassie Blue stained SDS-PAGE of LYPLA1 protein (His tag) (80R-1482) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €239.92
Size: 100 ug
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