Lysozyme antibody (20R-LR006)
Rabbit polyclonal Lysozyme antibody
Overview
Overview
| Synonyms | Polyclonal Lysozyme antibody, Anti-Lysozyme antibody, EC 3.2.1.17 antibody, Lyz antibody, 1 4 beta n acetylmuramidase c antibody, LZM antibody, Lysozyme C precursor antibody, lysosyme antibody, Lysozyme C antibody |
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| Specificity | Chicken lysozyme |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, IP, WB |
| Immunogen | Lysozyme antibody was raised in rabbit using full length protein corresponding to amino acids 1-129 of Hen Egg White lysozyme as the immunogen. |
Images
Western blot analysis using Lysozyme antibody (20R-LR006)
Western Blot of Lysozyme (Hen Egg White) antibody. Lane 1: Lysozyme (Hen Egg White). Load: 50 ng per lane. Lysozyme (Hen Egg White) antibody at 1:1,000 overnight at 4 deg C. HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).
Specifications
| Host | Rabbit |
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| Method of Purification | Lysozyme antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis. |
| Form & Buffer | Liquid in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% Sodium Azide |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:25,000; IP: 1:100; WB: 1:500-1:2,000; IHC: 1:500-1:2,000 |
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Storage & Safety
| Storage | Store at 4 deg Cfor short term storage and at-20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of ß-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme "c" is the most commonly studied form and source of the enzyme. |
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