mAdiponectin protein (His tag) (80R-1033)
Purified recombinant Mouse mAdiponectin protein
Overview
Overview
| Synonyms | GBP28 protein, , adipocyte complement-related protein protein, Acrp30 protein, Acdc protein, C1Q and collagen domain containing [Mus musculus] protein, Adiponectin protein, mAdiponectin protein, His-tagged protein, Mouse protein, 18- 247aa protein |
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| Species | Mouse |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of mAdiponectin protein (His tag) (80R-1033)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 18-247 amino acids: MGSSHHHHHH SSGLVPRGSH MEDDVTTTEE LAPALVPPPK GTCAGWMAGI PGHPGHNGTP GRDGRDGTPG EKGEKGDAGL LGPKGETGDV GMTGAEGPRG FPGTPGRKGE PGEAAYVYRS AFSVGLETRV TVPNVPIRFT KIFYNQQNHY DGSTGKFYCN IPGLYYFSYH ITVYMKDVKV SLFKKDKAVL FTYDQYQEKN VDQASGSVLL HLEVGDQVWL QVYGDGDHNG LYADNVNDST FTGFLLYHDT N |
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| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 27.2 kDa (251 aa), confirmed by MALDI-TOF. |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris, pH 8.0, containing 1mM DTT, and 10% glycerol. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Adiponectin is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. Mouse adiponectin shares about 83% amino acid identity with that human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Adiponectin is reduced in obese humans, and decreased level is associated with insulin resistance and hyperinsulinemia. Recombinant mouse adiponectin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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