MAPKSP1 protein (His tag) (80R-1566)
Purified recombinant Human MAPKSP1 protein
|Synonyms||Mitogen-activated protein kinase scaffold protein 1 protein, MAPKSP 1, MAPKSP 1 protein, MAPBP protein, MP1, MAPKSP-1 protein, MAPKSP1, MAPKSP-1, MAP2K1IP1 protein|
Coomassie Blue stained SDS-PAGE of MAPKSP1 protein (His tag) (80R-1566)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-124 amino acids: MGSSHHHHHH SSGLVPRGSH MADDLKRFLY KKLPSVEGLH AIVVSDRDGV PVIKVANDNA PEHALRPGFL STFALATDQG SKLGLSKNKS IICYYNTYQV VQFNRLPLVV SFIASSSANT GLIVSLEKEL APLFEELRQV VEVS|
|Grade & Purity||> 95% pure|
|Molecular Weight||15.8 kDa (144aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 5mM DTT and 100mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||MAPKSP1 (Mitogen-activated protein kinase scaffold protein 1) is a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Recombinant human MAPKSP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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