MASH1 antibody (10R-M106b)
Ratmonoclonal MASH1 antibody
Overview
Overview
| Synonyms | Monoclonal MASH1 antibody, Anti-MASH1 antibody, MASH 1 antibody, Achaete-scute homolog 1 antibody, MASH-1, MASH1, MASH-1 antibody, MASH 1 |
|---|---|
| Specificity | Mouse MASH1 |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, WB |
| Immunogen | MASH1 antibody was raised in Rat using E. coli-derived rmMash1 as the immunogen. |
Images
Immunofluorescent staining using MASH1 antibody (10R-M106b)
Ascl1 staining in a subpopulation of Type-1 stem cells (arrow) and Type-2 progenitors (arrowhead) in adult hippocampus using MASH1 (Ascl1) antibody at a dilution of 1:750. Imaging was performed on a Zeiss LSM510 confocal microscope. Image taken from Kim et al, Ascl1 (Mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain; Pubmed ID: 21483754 (See reference tab)
Specifications
| Host | Rat |
|---|---|
| Clone | 301733 |
| Isotype | IgG2a |
| Molecular Weight | 25.4 kDa (predicted detection band MW) |
| Form & Buffer | Lyophilized from a 0.2 u filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. |
Usage & Assay Information
| Usage Recommendations | ELISA: 0.5-1.0 ug/ml, WB: 1-2 ug/ml |
|---|
Storage & Safety
| Storage | Store at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles. |
|---|
General Information
| Biological Significance | The ASCL1 gene, is a basic helix-loop-helix (bHLH) transcription factor with a 40 AA helix-loop-helix motif (132 to 171) and a HLH N-terminal adjacent DNA-binding 11 AA basic motif (121-131). The protein also contains closely linked 15 AA polyAla (33-47) and 12 AA polyGln (51-62) regions. BasicHLH transcription factors are heterodimeric. Mash1 is essential for neuronal differentiation and specification within the nervous system. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product