MBP antibody (70R-10644)
Affinity purified Chicken polyclonal MBP antibody
Overview
Overview
| Synonyms | Polyclonal MBP antibody, Anti-MBP antibody, Myelin Basic Protein antibody |
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| Applications | ICC, IHC, WB |
| Immunogen | MBP antibody was raised in Chicken using a synthetic peptide (Human NP_002376 and Mouse NP_034907) / keyhole limpet hemocyanin (KLH) conjugate corresponding to the MBP gene product as the immunogen. |
Images
Immunofluorescent staining of MBP antibody (70R-10644)
A tissue section through an adult mouse brain showing MBP (red staining) of white matter tracts adjacent to the hippocampal formation. Green staining is autofluorescence from green fluorescent protein (GFP) expressed in this transgenic mouse.
Specifications
| Host | Chicken |
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| Method of Purification | MBP antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative. |
| Concentration | 100 ug/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:1000-1:2000; IHC: 1:1000-1:2000; WB: 1:2000-1:5000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury. |
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