MCEE protein (His tag) (80R-1514)
Purified recombinant Human MCEE protein
|Synonyms||GLOD2 protein, DL methylmalonyl CoA racemase protein, Glyoxalase domain containing 2, Methylmalonyl CoA epimerase protein, EC 22.214.171.124 protein|
Coomassie Blue stained SDS-PAGE of MCEE protein (His tag) (80R-1514)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||37-176 amino acids: MGSSHHHHHH SSGLVPRGSH MQVTGSVWNL GRLNHVAIAV PDLEKAAAFY KNILGAQVSE AVPLPEHGVS VVFVNLGNTK MELLHPLGRD SPIAGFLQKN KAGGMHHICI EVDNINAAVM DLKKKKIRSL SEEVKIGAHG KPVIFLHPKD CGGVLVELEQ A|
|Grade & Purity||> 90% pure|
|Molecular Weight||17.3 kDa (161aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||MCEE catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids, odd chain-length fatty acids, and other metabolites. This protein deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria may present in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma. Recombinant human MCEE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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