MCP1 antibody (10-M21A)

Mouse monoclonal MCP1 antibody

Synonyms Monoclonal MCP1 antibody, Anti-MCP1 antibody, JE antibody, Mouse antibody, MCP 1, MCP1, CCL2 antibody, Monocyte Chemotactic antibody-1 antibody, MCP-1 antibody, MCP-1, MCP-1 antibody, MCP 1 antibody, MCAF antibody
Specificity Human MCP1
Cross Reactivity To be determined by end-user
Applications ELISA, Inhibition, WB
Immunogen MCP1 antibody was raised in mouse using highly pure recombinant human MCP-1/MCAF as the immunogen.

Images

Monocyte Recruitment Involving MCP1

Release of monocytes from bone marrow during infection is driven by MCP1 (CCL2).

Specifications

Host Mouse
Clone M6110645
Isotype IgG1 kappa
Molecular Weight 8.6 kDa (predicted detection band MW)
Form & Buffer Lyophilized from PBS.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles.

General Information

Product Use Monoclonal MCP1 antibody specific for human MCP1/CCL2 for use in ELISA, Western Blot and Inhibition studies.
Biological Significance MCP1/CCL2 is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. MCP1 augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. MCP1 may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. MCP1 binds to CCR2 and CCR4 and is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family. MCP1 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection.
Expression Platelet derived growth factor is a major inducer of CCL2 gene. To become activated MCP1 protein has to be cleaved by metalloproteinase MMP-12. MCP1 is a monomeric polypeptide, with a molecular weight of approximately 13 kDa. MCP1 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. MCP1 is primarily secreted by monocytes, macrophages and dendritic cells. In the bone, MCP1 is expressed by mature osteoclasts and osteoblasts. MCP1 is also expressed by neurons, astrocytes and microglia. The expression of CCL2 in neurons is mainly found in the cerebral cortex, globus pallidus, hippocampus, paraventricular and supraoptic hypothalamic nuclei, lateral hypothalamus, substantia nigra, facial nuclei, motor and spinal trigeminal nuclei, gigantocellular reticular nucleus and in Purkinje cells in the cerebellum.
Implications in Disease MCP1 is implicated in pathogeneses of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. MCP1 is also is involved in the neuroinflammatory processes that takes place in the various diseases of the central nervous system (CNS), which are characterized by neuronal degeneration. CCL2 expression in glial cells is increased in epilepsy, brain ischemia Alzheimer’s disease experimental autoimmune encephalomyelitis (EAE), and traumatic brain injury.
Sequence Information MCP1 and many other CC chemokines are located on chromosome 17 (17q11.2-q21.1). The gene span is 1,927 bases and the CCL2 gene resides on the Watson (plus) strand. The CCL2 gene has three exons and two introns. The CCL2 protein precursor contains a signal peptide of 23 amino acids. In turn, the mature CCL2 is 76 amino acids long. The CCL2 predicted weight is 11.025 kiloDaltons (kDa).
Post-Translational Modifications MCP1 undergoes glycosylation at Asn37. Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Database Links Entrez Gene: 6347 Human, Omim: 158105 Human, SwissProt: P13500 Human, Unigene: 303649 Human

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Monocyte Recruitment Involving MCP1 | Release of monocytes from bone marrow during infection is driven by MCP1 (CCL2).

Availability: In stock

Price: €465.72
Size: 500 ug
OR
Shipping
View Our Distributors