MCSF protein (30R-2218)

Purified recombinant Human MCSF protein

Synonyms CSF-1 protein, monocyte colony stimulating factor protein, MCSF protein, Macrophage colony stimulating factor protein
Species Human
Protein Type Recombinant
Applications Functional Assay
Assay Information The ED50 of this protein, as measured by 2E8 cell proliferation assay, is less than or equal to 1.6 ng/ml. This corresponds to a specific activity of greater than or equal to 6.3 x 10e5 Units/mg.

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Functional Assay using MCSF protein (30R-2218)

Proliferation of M-NFS-60 cells induced by Human M-CSF protein.

Specifications

Expression System Insect cells
Grade & Purity > 98% pure
Method of Purification MCSF protein was purified by affinity chromatography.
Form & Buffer Supplied as a sterile liquid with phosphate-buffered saline 1% BSA,0.22 um filtered.

Usage & Assay Information

Bioactivity The bioactivity of this protein, as determined in acell proliferation assay on M-NFS-60 cells, is 3ng/ml. This corresponds to a specific activity of 3.3 X 10 5 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Human Macrophage Colony Stimulating Factor is a ~36 kDa disulfide-linked homodimer produced by a variety of cells including fibroblasts, macrophages, bone marrow stromal cells and endothelial cells. M-CSF regulates macrophage survival, proliferation and differentiation. M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages.

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  • Functional Assay using MCSF protein (30R-2218) | Proliferation of M-NFS-60 cells induced by Human M-CSF protein.

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