MCSF protein (30R-2218)
Purified recombinant Human MCSF protein
Overview
Overview
| Synonyms | CSF-1 protein, monocyte colony stimulating factor protein, MCSF protein, Macrophage colony stimulating factor protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | Functional Assay |
| Assay Information | The ED50 of this protein, as measured by 2E8 cell proliferation assay, is less than or equal to 1.6 ng/ml. This corresponds to a specific activity of greater than or equal to 6.3 x 10e5 Units/mg. |
Images
Functional Assay using MCSF protein (30R-2218)
Proliferation of M-NFS-60 cells induced by Human M-CSF protein.
Specifications
| Expression System | Insect cells |
|---|---|
| Grade & Purity | > 98% pure |
| Method of Purification | MCSF protein was purified by affinity chromatography. |
| Form & Buffer | Supplied as a sterile liquid with phosphate-buffered saline 1% BSA,0.22 um filtered. |
Usage & Assay Information
| Bioactivity | The bioactivity of this protein, as determined in acell proliferation assay on M-NFS-60 cells, is 3ng/ml. This corresponds to a specific activity of 3.3 X 10 5 Units/mg. |
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Storage & Safety
| Storage | Store at -70 deg C or less. |
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| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Human Macrophage Colony Stimulating Factor is a ~36 kDa disulfide-linked homodimer produced by a variety of cells including fibroblasts, macrophages, bone marrow stromal cells and endothelial cells. M-CSF regulates macrophage survival, proliferation and differentiation. M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages. |
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