MCSF protein (Mouse) (30R-2232)

Purified recombinant Mouse MCSF protein

Synonyms Macrophage colony stimulating factor protein, CSF-1 protein, MCSF protein, monocyte colony stimulating factor protein
Specificity Mouse
Species Mouse
Protein Type Recombinant
Applications Functional Assay
Assay Information The recombinant mouse IL-27 (EBI3/p28) has been reported useful for bioassay.

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Functional Assay using MCSF protein (Mouse) (30R-2232)

Proliferation of M-NFS-60 cells in response to Mouse M-CSF protein.

Specifications

Expression System Insect cells
Grade & Purity > 98% pure
Method of Purification MCSF protein was purified by affinity chromatography.
Molecular Weight 44.5 kDa
Form & Buffer Supplied as a sterile liquid with phosphate buffered saline with 1% BSA, pH 7.2 0.22 um filtered.

Usage & Assay Information

Bioactivity The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10 ng/ml. This corresponds to a specific activity of greater than or equal to 1 x 10^5 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Macrophage colony-stimulating factor is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms induces dimerization of the receptor followed by internalization and degradation of the complex.

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  • Functional Assay using MCSF protein (Mouse) (30R-2232) | Proliferation of M-NFS-60 cells in response to Mouse M-CSF protein.

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