MCSF protein (Mouse) (30R-2232)
Purified recombinant Mouse MCSF protein
Overview
Overview
| Synonyms | Macrophage colony stimulating factor protein, CSF-1 protein, MCSF protein, monocyte colony stimulating factor protein |
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| Specificity | Mouse |
| Species | Mouse |
| Protein Type | Recombinant |
| Applications | Functional Assay |
| Assay Information | The recombinant mouse IL-27 (EBI3/p28) has been reported useful for bioassay. |
Images
Functional Assay using MCSF protein (Mouse) (30R-2232)
Proliferation of M-NFS-60 cells in response to Mouse M-CSF protein.
Specifications
| Expression System | Insect cells |
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| Grade & Purity | > 98% pure |
| Method of Purification | MCSF protein was purified by affinity chromatography. |
| Molecular Weight | 44.5 kDa |
| Form & Buffer | Supplied as a sterile liquid with phosphate buffered saline with 1% BSA, pH 7.2 0.22 um filtered. |
Usage & Assay Information
| Bioactivity | The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10 ng/ml. This corresponds to a specific activity of greater than or equal to 1 x 10^5 Units/mg. |
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Storage & Safety
| Storage | Store at -70 deg C or less. |
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| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Macrophage colony-stimulating factor is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms induces dimerization of the receptor followed by internalization and degradation of the complex. |
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