MHC Class I antibody (biotin) (61R-1629)

Mouse monoclonal MHC Class I antibody (biotin)

Synonyms Monoclonal MHC Class I antibody, Anti-MHC Class I antibody, MHCI antibody, MHC I antibody, MHC Class I antibody, H2K-b antibody, H2-Kb antibody, Anti MHC Class I antibody, H2-Kb antibody, H2K-b antibody, MHCI antibody, MHC I antibody, H2Kb antibody, H2Kb antibody
Specificity Mouse
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using MHC Class I antibody (biotin) (61R-1629)

Staining of BALB/c (left) and C57BL/6 (right) splenocytes with 0.125 ug of Mouse IgG2a kappa Isotype Control (biotin) (blue histogram) or 0.125 ug of MHC Class I antibody (H-2Kd) (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone SF1-1.1.1
Isotype IgG2a
Tag/Conjugate Biotin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.06 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes has not been observed.

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Images

  • Flow Cytometric analysis using MHC Class I antibody (biotin) (61R-1629) | Staining of BALB/c (left) and C57BL/6 (right) splenocytes with 0.125 ug of Mouse IgG2a kappa Isotype Control (biotin) (blue histogram) or 0.125 ug of MHC Class I antibody (H-2Kd) (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.

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Price From: Price: €77.71
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