MHC Class I antibody (FITC) (61R-1165)

Mouse monoclonal MHC Class I antibody (FITC)

Synonyms Monoclonal MHC Class I antibody, Anti-MHC Class I antibody, MHCI antibody, MHC Class I antibody, H2Db antibody, H2Db antibody, Anti MHC Class I antibody, MHCI antibody
Specificity Mouse
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using MHC Class I antibody (FITC) (61R-1165)

Staining of C57BL/6 splenocytes with 0.25 ug of Mouse IgG2a kappa Isotype Control (FITC) (open histogram) or 0.25 ug of MHC Class I antibody (H-2Db) (FITC) (filled histogram). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone 28-14-8
Isotype IgG2a kappa
Tag/Conjugate FITC
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: =0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2Db, and cross-reacts with H-2L, H-2Dq and/or H-2Lq. Binding of 28-14-8 to the alpha 3 domain of H-2L is not dependent on beta2-microglobulin.

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Images

  • Flow Cytometric analysis using MHC Class I antibody (FITC) (61R-1165) | Staining of C57BL/6 splenocytes with 0.25 ug of Mouse IgG2a kappa Isotype Control (FITC) (open histogram) or 0.25 ug of MHC Class I antibody (H-2Db) (FITC) (filled histogram). Total viable cells were used for analysis.

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Price From: Price: €149.69

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Price From: Price: €149.69
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