MHC Class I H2Kk antibody (biotin) (61R-1624)

Mouse monoclonal MHC Class I H2Kk antibody (biotin)

Synonyms Monoclonal MHC Class I H2Kk antibody, Anti-MHC Class I H2Kk antibody, H2-Kk antibody, H2Kk antibody, H2K-k antibody, MHC Class I HKk 2 antibody, MHC Class I HKk 2, MHC Class I H2Kk, MHC Class I HKk-2 antibody, MHC I antibody, MHC Class I HKk-2
Specificity Mouse
Applications FC, IHC
Assay Information test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using MHC Class I H2Kk antibody (biotin) (61R-1624)

Staining of splenocytes from B10.BR (left) and Balb/c (right) mice with 0.125 ug of Mouse IgG1 kappa Isotype Control (biotin) (blue histogram) or 0.06 ug of MHC Class I antibody H-2Kk (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone AF3-12.1.3
Isotype IgG1
Tag/Conjugate Biotin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance This AF3-12.1.3 monoclonal antibody reacts with the H-2Kk MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes has not been observed.

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Images

  • Flow Cytometric analysis using MHC Class I H2Kk antibody (biotin) (61R-1624) | Staining of splenocytes from B10.BR (left) and Balb/c (right) mice with 0.125 ug of Mouse IgG1 kappa Isotype Control (biotin) (blue histogram) or 0.06 ug of MHC Class I antibody H-2Kk (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.

Availability: In stock

Price: €208.59

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Price: €208.59
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