MHC Class I H2Kk antibody (biotin) (61R-1624)
Mouse monoclonal MHC Class I H2Kk antibody (biotin)
Overview
Overview
| Synonyms | Monoclonal MHC Class I H2Kk antibody, Anti-MHC Class I H2Kk antibody, H2-Kk antibody, H2Kk antibody, H2K-k antibody, MHC Class I HKk 2 antibody, MHC Class I HKk 2, MHC Class I H2Kk, MHC Class I HKk-2 antibody, MHC I antibody, MHC Class I HKk-2 |
|---|---|
| Specificity | Mouse |
| Applications | FC, IHC |
| Assay Information | test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using MHC Class I H2Kk antibody (biotin) (61R-1624)
Staining of splenocytes from B10.BR (left) and Balb/c (right) mice with 0.125 ug of Mouse IgG1 kappa Isotype Control (biotin) (blue histogram) or 0.06 ug of MHC Class I antibody H-2Kk (biotin) (purple histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.
Specifications
| Host | Mouse |
|---|---|
| Clone | AF3-12.1.3 |
| Isotype | IgG1 |
| Tag/Conjugate | Biotin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.25 ug/test |
|---|
Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
|---|
General Information
| Biological Significance | This AF3-12.1.3 monoclonal antibody reacts with the H-2Kk MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes has not been observed. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product