MHC Class II antibody (10R-6527)

Rat monoclonal MHC Class II antibody

Synonyms Monoclonal MHC Class II antibody, Anti-MHC Class II antibody, MHC II antibody, I-A/E antibody, IE antibody, IE antibody, Anti MHC Class II antibody, MHC II antibody, MHCII antibody, I-A/E antibody, MHCCII antibody, IA antibody, IA antibody, MHCII antibody, MHC Class II antibody, MHCCII antibody, IA/IE antibody, IA/IE antibody
Specificity Mouse
Applications FC, IP, WB
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Images

Flow Cytometric analysis using MHC Class II antibody (10R-6527)

Staining of C57BL/6 splenocytes with 0.06 ug of Rat IgG2b K Isotype Control (open histogram) or 0.06 ug of MHC class II antibody (I-A/I-E) Purified (filled histogram) followed by Anti-Rat IgG (FITC). Total viable cells were used for analysis.

Specifications

Host Rat
Clone M5/114.15.2
Isotype IgG2b kappa
Method of Purification MHC Class II antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.5 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins. It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

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Images

  • Flow Cytometric analysis using MHC Class II antibody (10R-6527) | Staining of C57BL/6 splenocytes with 0.06 ug of Rat IgG2b K Isotype Control (open histogram) or 0.06 ug of MHC class II antibody (I-A/I-E) Purified (filled histogram) followed by Anti-Rat IgG (FITC). Total viable cells were used for analysis.

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Price From: Price: €85.89

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Price From: Price: €85.89
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