MHC Class II antibody (biotin) (61R-1539)

Mouse monoclonal MHC Class II antibody (biotin)

Synonyms Monoclonal MHC Class II antibody, Anti-MHC Class II antibody, MHCII antibody, MHCII antibody, Anti MHC Class II antibody, MHC Class II antibody, MHCCII antibody, MHCII antibody, MHCII antibody, MHCCII antibody
Specificity Human
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using MHC Class II antibody (biotin) (61R-1539)

Staining of rat splenocytes with MHC Class II antibody (FITC) (left), (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone HIS19
Isotype IgG1
Tag/Conjugate Biotin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.125 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n. The rat MHC class II, RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages.

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Images

  • Flow Cytometric analysis using MHC Class II antibody (biotin) (61R-1539) | Staining of rat splenocytes with MHC Class II antibody (FITC) (left), (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Availability: In stock

Price: €251.12
Size: 100 ug
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