MHC Class II antibody (biotin) (61R-1590)

Rat monoclonal MHC Class II antibody (biotin)

Synonyms Monoclonal MHC Class II antibody, Anti-MHC Class II antibody, IE antibody, IA antibody, IA antibody, I-A/E antibody, MHCCII antibody, MHCII antibody, IA/IE antibody, IE antibody, MHC II antibody, IA/IE antibody, Anti MHC Class II antibody, MHCII antibody, MHCCII antibody, I-A/E antibody, MHC Class II antibody, MHC II antibody
Specificity Mouse
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

Images

Flow Cytometric analysis using MHC Class II antibody (biotin) (61R-1590)

Staining of C57BL/6 splenocytes with 0.015 ug of Rat IgG2b K Isotype Control (biotin) (open histogram) or 0.015 ug of MHC class II antibody (I-A/I-E) (biotin) followed by Streptavidin (PE) (filled histogram). Total viable cells were used for analysis.

Specifications

Host Rat
Clone M5/114.15.2
Isotype IgG2b kappa
Tag/Conjugate Biotin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins. It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

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Images

  • Flow Cytometric analysis using MHC Class II antibody (biotin) (61R-1590) | Staining of C57BL/6 splenocytes with 0.015 ug of Rat IgG2b K Isotype Control (biotin) (open histogram) or 0.015 ug of MHC class II antibody (I-A/I-E) (biotin) followed by Streptavidin (PE) (filled histogram). Total viable cells were used for analysis.

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Price From: Price: €125.97

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Price From: Price: €125.97
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