MHC Class II antibody (FITC) (61R-1137)

Rat monoclonal MHC Class II antibody (FITC)

Synonyms Monoclonal MHC Class II antibody, Anti-MHC Class II antibody, MHC II antibody, IA antibody, MHCCII antibody, I-A/E antibody, I-A/E antibody, Anti MHC Class II antibody, IE antibody, MHCII antibody, IE antibody, MHC Class II antibody, IA/IE antibody, MHCII antibody, MHCCII antibody, IA/IE antibody, IA antibody, MHC II antibody
Specificity Mouse
Applications FC
Assay Information ug size: has been tested by flow cytometric analysis of human peripheral blood leukocytes and should be used at the same concentration as the experimental antibody.

Images

Flow Cytometric analysis using MHC Class II antibody (FITC) (61R-1137)

Staining of C57BL/6 splenocytes with CD3e antibody and 0.06 ug of Rat IgG2b K Isotype Control (FITC) (left) or 0.06 ug of MHC class II antibody (I-A/I-E) (FITC) (right). Cells in the lymphocyte gate were used for analysis.

Specifications

Host Rat
Clone M5/114.15.2
Isotype IgG2b kappa
Tag/Conjugate FITC
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins. It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

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Images

  • Flow Cytometric analysis using MHC Class II antibody (FITC) (61R-1137) | Staining of C57BL/6 splenocytes with CD3e antibody and 0.06 ug of Rat IgG2b K Isotype Control (FITC) (left) or 0.06 ug of MHC class II antibody (I-A/I-E) (FITC) (right). Cells in the lymphocyte gate were used for analysis.

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Price From: Price: €125.97

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Price From: Price: €125.97
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