MHC class II antibody (FITC) (61R-1157)
Mouse monoclonal MHC class II antibody (FITC)
Overview
Overview
| Synonyms | Monoclonal MHC class II antibody, Anti-MHC class II antibody, IE-k antibody, IE-k antibody, Iek antibody, I-E(k) antibody, MHC II antibody, Iek antibody, Anti MHC class II antibody, MHCCII antibody, MHCCII antibody, MHCII antibody, MHC class II antibody, I-E(k) antibody, MHCII antibody, MHC II antibody |
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| Specificity | Mouse, Rat |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest |
Images
Flow Cytometric analysis using MHC class II antibody (FITC) (61R-1157)
Staining of BALB/c splenocytes with 0.125 ug of Mouse IgG2a kappa Isotype Control (FITC) (open histogram) or 0.125 ug of MHC class II antibody (FITC) (filled histogram). Total viable cells were used for analysis.
Specifications
| Host | Mouse |
|---|---|
| Clone | 14-4-4S |
| Isotype | IgG2a kappa |
| Tag/Conjugate | FITC |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2, H-2p and H-2r. H-2b, H-2f and H-2s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat. |
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