MHC Class II antibody (PE) (61R-1285)
Mouse monoclonal MHC Class II antibody (PE)
Overview
Overview
| Synonyms | Monoclonal MHC Class II antibody, Anti-MHC Class II antibody, MHCCII antibody, MHCII antibody, MHCII antibody, MHCII antibody, Anti MHC Class II antibody, MHCII antibody, MHCCII antibody, MHC Class II antibody |
|---|---|
| Specificity | Human |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. |
Images
Flow Cytometric analysis using MHC Class II antibody (PE) (61R-1285)
Staining of rat splenocytes with Mouse IgG1 kappa Isotype Control (FITC) (left, open histogram) or Mouse IgG1 kappa Isotype Control (PE) (right, open histogram) and MHC Class II antibody (FITC) (left, filled histogram) or MHC Class II antibody (PE) (right, filled histogram). Total viable cells were used for analysis.
Specifications
| Host | Mouse |
|---|---|
| Clone | HIS19 |
| Isotype | IgG1 |
| Tag/Conjugate | PE |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 5 ul (0.125 ug)/test |
Usage & Assay Information
| Usage Recommendations | FC: 5 ul (0.125 ug)/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n. The rat MHC class II, RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages. |
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