MHC Class II antibody (PE) (61R-1340)
Rat monoclonal MHC Class II antibody (PE)
Overview
Overview
| Synonyms | Monoclonal MHC Class II antibody, Anti-MHC Class II antibody, MHCCII antibody, IA antibody, IA/IE antibody, IE antibody, MHC II antibody, MHCCII antibody, MHC II antibody, I-A/E antibody, MHCII antibody, MHC Class II antibody, IA/IE antibody, MHCII antibody, IA antibody, Anti MHC Class II antibody, IE antibody, I-A/E antibody |
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| Specificity | Mouse |
| Applications | FC |
Images
Flow Cytometric analysis using MHC Class II antibody (PE) (61R-1340)
Staining of C57BL/6 splenocytes with CD45R antibody (FITC) and staining buffer (Autofluorescence) (left) or 0.0075 ug of MHC class II antibody (I-A/I-E) (PE) (right). Total viable cells were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | M5/114.15.2 |
| Isotype | IgG2b kappa |
| Tag/Conjugate | PE |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
Usage & Assay Information
| Usage Recommendations | FC: 20 ul (0.5 ug)/100 ul blood (or/1 million cells in 100 ul total staining volume) |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins. It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes. |
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