MHC class II antibody (PE) (61R-1391)
Mouse monoclonal MHC class II antibody (PE)
Overview
Overview
| Synonyms | Monoclonal MHC class II antibody, Anti-MHC class II antibody, Iek antibody, MHC II antibody, MHCII antibody, MHCII antibody, MHC II antibody, MHCCII antibody, MHCCII antibody, MHC class II antibody, IE-k antibody, IE-k antibody, Iek antibody, I-E(k) antibody, I-E(k) antibody, Anti MHC class II antibody |
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| Specificity | Mouse, Rat |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest |
Images
Flow Cytometric analysis using MHC class II antibody (PE) (61R-1391)
Staining of mouse splenocytes with MHC class II antibody (FITC) (left), (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.
Specifications
| Host | Mouse |
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| Clone | 14-4-4S |
| Isotype | IgG2a kappa |
| Tag/Conjugate | PE |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 200 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: =0.125 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2, H-2p and H-2r. H-2b, H-2f and H-2s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat. |
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