MHC class II antibody (PE) (61R-1391)

Mouse monoclonal MHC class II antibody (PE)

Synonyms Monoclonal MHC class II antibody, Anti-MHC class II antibody, Iek antibody, MHC II antibody, MHCII antibody, MHCII antibody, MHC II antibody, MHCCII antibody, MHCCII antibody, MHC class II antibody, IE-k antibody, IE-k antibody, Iek antibody, I-E(k) antibody, I-E(k) antibody, Anti MHC class II antibody
Specificity Mouse, Rat
Applications FC
Assay Information A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

Images

Flow Cytometric analysis using MHC class II antibody (PE) (61R-1391)

Staining of mouse splenocytes with MHC class II antibody (FITC) (left), (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone 14-4-4S
Isotype IgG2a kappa
Tag/Conjugate PE
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations FC: =0.125 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2, H-2p and H-2r. H-2b, H-2f and H-2s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat.

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Images

  • Flow Cytometric analysis using MHC class II antibody (PE) (61R-1391) | Staining of mouse splenocytes with MHC class II antibody (FITC) (left), (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

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Price From: Price: €122.70
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