MIF protein (30R-2203)

Purified recombinant Human MIF protein

Synonyms L-dopachrome tautomerase protein, L-dopachrome isomerase protein, GIF protein, Macrophage migration inhibitory factor protein, Glycosylation-inhibiting factor protein
Species Human
Protein Type Recombinant
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Specifications

Expression System E.coli
Grade & Purity > 97% pure
Method of Purification MIF protein was purified by affinity chromatography.
Molecular Weight 12.5 kDa
Form & Buffer Supplied as a sterile liquid with phosphate buffered saline, pH 7.2,1.0% BSA. 0.22 um filtered.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.5 ug/test
Bioactivity The optimal range of activity found in an agarosemicrodroplet assay using human U937 cells is 0.25-1ug/ml.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance MIF is an inflammatory mediator in response to bacterial lipopolysaccharide. MIF stimulates IL-1, IL-8 and MMP expression on fibroblasts. MIF stimulates NO and TNF- alpha production on macrophages. The secretion of MIF is regulated by steroids and MIF counter-regulates the immunosuppressive effects of steroids on immune response and cytokine induction. Therefore MIF plays an important role in endotoxemia and septic shock. The expression of MIF activity is correlated with delayed hypersensitivity and cellular immunity. It has been reported that the expression of MIF is significantly increased in cardiomyocytes in chronic hypoxia and the expression of MIF is implicated in cancer pathogenesis. Human MIF is a non-glycosylated polypeptide consisting of 115 amino acids with a molecular weight of 12.5 kDa.

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