MIF protein (His tag) (80R-1007)
Purified recombinant Human MIF protein
Overview
Overview
| Synonyms | Phenylpyruvate tautomerase protein, Macrophage migration inhibitory factor Glycosylation inhibiting factor protein, , MIF protein protein, Macrophage migration inhibitory factor protein, Glycosylation-inhibiting factor protein, MIF protein, EC 5.3.2.1 protein, GLIF protein, GIF protein, MMIF protein, MIF (1-114aa) protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of MIF protein (His tag) (80R-1007)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMPMF IVNTNVPRAS VPDGFLSELT QQLAQATGKP PQYIAVHVVP DQLMAFGGSS EPCALCSLHS IGKIGGAQNR SYSKLLCGLL AERLRISPDR VYINYYDMNA ANVGWNNSTF A |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 16.6 kDa (151 aa), confirmed by MALDI-TOF. |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in PBS, pH 7.4. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
|---|---|
| Endotoxin Levels | < 1.0 EU per ug of protein (determined by LAL method) |
General Information
| Biological Significance | The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. |
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