Polyclonal MNAT1 antibody, Anti-MNAT1 antibody, CAP35 antibody, MAT1 antibody, RNF66 antibody, CDK-activating kinase assembly factor MAT1 antibody, CDK7/cyclin-H assembly factor antibody, Cyclin-G1-interacting protein antibody, Menage a trois antibody, RING finger protein 66 antibody, RING finger protein MAT1 antibody, p35 antibody, p36 antibody
Specificity
Recognizes endogenous levels of MNAT1 protein
Cross Reactivity
Mouse, Rat, Bovine, Pig
Applications
IHC, IP, WB
Immunogen
MNAT1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MNAT1 as the immunogen
Western blot analysis using MNAT1 antibody (70R-50080)
Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.
MNAT1 antibody
Western blot analysis using MNAT1 antibody (70R-50080)
Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.
Immunohistochemical analysis using MNAT1 antibody (70R-50080)
Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Specifications
Host
Rabbit
Method of Purification
MNAT1 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
MNAT1, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II.