Mouse IgG (Fc) (FITC) (65C-CH1017)

Purified Mouse IgG (Fc) FITC conjugate

Synonyms Mouse Immunoglobulin G (Fc) (FITC)
Species Mouse
Applications IF

Images

SDS-PAGE analysis of Mouse IgG (Fc) (FITC) (65C-CH1017)

Lane 1: 5 uL MW Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment Fluorescein Conjugated. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Specifications

Method of Purification Mouse IgG (Fc) (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Tag/Conjugate FITC
Form & Buffer Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fc regions of IgGs bear a highly conserved N-glycosylation site. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and alpha-2,6-linked sialic acid residues.

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Images

  • SDS-PAGE analysis of Mouse IgG (Fc) (FITC) (65C-CH1017) |  Lane 1: 5 uL MW Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment Fluorescein Conjugated. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Availability: In stock

Price: €465.72
Size: 1 mg
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