Mouse Leptin protein (30R-1100)
Purified recombinant Mouse Leptin protein
Overview
Overview
| Synonyms | Obese protein protein, Obesity factor protein, LEP protein, OB protein, , Leptin Mouse protein, Leptin protein, OBS protein |
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| Species | Mouse |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of Mouse Leptin protein (30R-1100)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 16 kDa (147 aa), confirmed by MALDI-TOF. |
| Form & Buffer | Supplied as a liquid in PBS, pH 7.4. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. Originally discovered in the mouse, the cDNA for leptin has been so far cloned from a variety of species including human, rat, cow, chicken, pig, sheep and dog. Recombinant mouse leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer |
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