Mouse PRMT1 protein (isoform 2) (MBP tag) (80R-1041)
Purified recombinant Mouse PRMT1 protein (isoform 2) (His-MBP tag)
Overview
Overview
| Synonyms | Mrmt 1 protein, cerevisiae like 2 protein, Interferon receptor 1 bound protein 4 protein, HMT2 protein, HMT1 (hnRNP methyltransferase S protein, Protein arginine N-methytransferase 1 protein, cerevisiae) protein, HMT1 hnRNP methyltransferase protein, IR1B 4 protein, HMT1 hnRNP methyltransferase like 2 protein, Human mRNA for suppressor for yeast mutant complete cds protein, Heterogeneous nuclear ribonucleoproteins methyltransferase like2 protein, R1B4 protein, HMT 2 protein, PRMT-1 protein, cerevisiae) like 2 protein, IR1 B4 protein, HRMT1L 2 protein, Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 protein, S protein, Mrmt1 protein, AW214366 protein, PRMT1, Protein arginine N-methyltransferase 1 ANM 1 protein, Protein arginine N methyltransferase1 protein, PRMT-1, HCP 1 protein, Human mRNA for suppressor for yeast mutant protein, Protein arginine N methyltransferase 1 protein, PRMT 1, Mrmt1 protein, Interferon receptor 1bound protein 4 protein, PRMT1 protein, PRMT 1 protein, PRMT1 isoform2 protein, HRMT1 L2 protein, PRMT 1 protein, HMT1 hnRNP methyltransferase like 2 (S protein, Hrmt112 protein, Interferon receptor 1 bound protein4 protein, |
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| Species | Mouse |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
| Assay Information | One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37 DEG C. |
Images
Coomassie Blue stained SDS-PAGE of Mouse PRMT1 protein (isoform 2) (MBP tag) (80R-1041)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-353 amino acids: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR |
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| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 84 kDa (750 aa) |
| Tag/Conjugate | MBP tag |
| Form & Buffer | Supplied as a liquid in 40mM Tris, pH 8.0, containing 100mM NaCl, 4mM MgCl2, 2mM DTT, and 40% glycerol. |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Bioactivity | 10,000unit/mg |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Protein arginine N-methyltransferase 1 (PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Mouse PRMT1 (AAF37293) shares 99.9% sequence identity with the human proteins (NP_938074). Recombinant mouse PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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