MRCK alpha antibody (70R-12679)

Affinity purified Rabbit polyclonal MRCK alpha antibody

Synonyms Polyclonal MRCK alpha antibody, Anti-MRCK alpha antibody, myotonic dystrophy protein kinase-like alpha antibody, DKFZp686P1738 antibody, DKFZp686L1738 antibody, KIAA0451 antibody, PK428 antibody, MRCKA antibody, CDC42BPA antibody, ser-thr protein kinase PK428 antibody, ser-thr protein kinase related to the myotonic dystrophy protein kinase antibody, MRCK antibody, FLJ23347 antibody, DMPK-like alpha antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen MRCK alpha antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 373 and 564 of MRCK alpha

Images

Immunofluorescent staining using MRCK alpha antibody (70R-12679)

Immunofluorescence analysis of methanol-fixed HCT116, using MRCK alpha antibody at 1:500 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification MRCK alpha antibody was purified by Protein A chromatography
Molecular Weight 186 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 2 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using MRCK alpha antibody (70R-12679)</font></div>     | Immunofluorescence analysis of methanol-fixed HCT116, using MRCK alpha antibody at 1:500 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using MRCK alpha antibody (70R-12679)</font></div>     | Immunohistochemical staining of paraffin-embedded SW480 xenograft using MRCK alpha antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using MRCK alpha antibody (70R-12679)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:H1299) using a 7.5 % SDS PAGE gel and MRCK alpha antibody at a dilution of 1:1500

Availability: In stock

Price: €323.11
Size: 100 ul
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