msrB protein (His tag) (80R-1783)
Purified recombinant E.coli msrB protein
Overview
Overview
| Synonyms | Methionine sulfoxide reductase B protein, ECK1776 protein, JW1767 protein, yeaA protein |
|---|---|
| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of msrB protein (His tag) (80R-1783)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-137 amino acids: MGSSHHHHHH SSGLVPRGSH MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFY EPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 17.6 kDa (157aa) confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E. coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possesses a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes. Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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