Mumps IgG ELISA Kit (55R-RE56641)
ELISA kit for detection of Mumps IgG in the research laboratory
Overview
Overview
| Synonyms | Mumps ELISA Kit |
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| Applications | ELISA |
| Assay Information | The Mumps IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Mumps antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Mumps antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color. |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user |
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Storage & Safety
| Storage | Store at 4 deg C. |
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| Biohazard Information | All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as pote |
General Information
| Biological Significance | Mumps (Parotitis) is a common contagious disease with relatively moderate symptoms during childhood, but increasing complications, when adults are infected. The causative agent of mumps is a virus of the Paramyxoviridae family. The virus normally infects children at the age of 4 to 10. The infection is mainly transmitted by the airborne route, but is also spread by various objects contaminated by the patient saliva. |
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