NAP2 antibody (70R-NG001)

Affinity purified Goat polyclonal NAP2 antibody

Synonyms Polyclonal NAP2 antibody, Anti-NAP2 antibody, NAP 2 antibody, NAP 2, NAP-2, PBP antibody, NAP2, NAP-2 antibody, NAP-2 antibody, CTAP-III antibody, parent molecule antibody, precursor antibody, Neutrophil Activating antibody-2 antibody, CXCL7 antibody
Specificity Human NAP2
Cross Reactivity To be determined by end-user
Applications ELISA, Inhibition, WB
Immunogen NAP2 antibody was raised in goat using highly pure recombinant hNAP-2 as the immunogen.

Specifications

Host Goat
Molecular Weight 7.6 kDa (predicted detection band MW)
Form & Buffer Lyophilized from PBS, pH 7.2.

Usage & Assay Information

Usage Recommendations Optimal conditions to be detrmined by user. Indirect: To detect hNAP-2 byIndirect ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. Sandwich: To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech?s Biotinylated Anti-Human NAP-2 (500-P03GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. To detect hNAP-2 by WB analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Storage & Safety

Storage Store at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance NAP-2 is a CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is produced in leukocytes by enzymatic processing of a precursor called platelet basic protein (PBP). NAP-2 chemoattracts and activates neutrophils.

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Availability: In stock

Price: €211.69
Size: 50 ug
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