NAPA protein (His tag) (80R-1470)

Purified recombinant Human NAPA protein

Synonyms SNAP-alpha, N-ethylmaleimide-sensitive factor attachment protein alpha protein, SNAPA protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of NAPA protein (His tag) (80R-1470)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-295 amino acids: MGSSHHHHHH SSGLVPRGSH MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 35.3 kDa(315aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl Buffer, pH 7.5, containing 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues. Recombinant human NAPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of NAPA protein (His tag) (80R-1470) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €239.92
Size: 100 ug
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