Immunofluorescent staining using NARS antibody (10R-1221)
Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NARS antibody.
NARS antibody
Immunofluorescent staining using NARS antibody (10R-1221)
Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NARS antibody.
Western Blot analysis of immunized recombinant protein using NARS antibody (10R-1221)
Specifications
Host
Mouse
Clone
NARS2G5
Isotype
IgG1
Method of Purification
NARS antibody was purified using protein G column chromatography from culture supernatant of hybridoma
cultured in a medium containing bovine IgG-depleted
(approximately 95%) fetal bovine serum.
Form & Buffer
NARS antibody in PBS (3.0 mM KCl, 1.5 mM KH2PO4 , 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information
This antibody solution contains sodium azide (NaN )
as a preservative. There is a potential hazard that
NaN reacts with copper or lead to produce an explosive
compound. For safe disposal, the vial has to
be washed thoroughly with water. It is strongly adv
General Information
Biological Significance
Aminoacy1-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginy1-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginy1-tRNA synthetases.