NCS1 protein (His tag) (80R-1291)
Purified recombinant Human NCS1 protein
|Synonyms||NCS-1 protein, NCS 1, NCS1 protein, FREQ protein, Frequenin-like protein protein, NCS1, Neuronal calcium sensor 1 Flup protein, NCS 1 protein, Neuronal Calcium Sensor 1., Frequenin homolog protein, frequenin (Drosophila) homolog protein, NCS-1, Freq protein, FLUP protein|
Coomassie Blue stained SDS-PAGE of NCS1 protein (His tag) (80R-1291)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-190 amino acids: MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV LEHHHHHH|
|Grade & Purity||> 95% pure|
|Molecular Weight||22.9 kDa (198aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Neuronal calcium sensor 1, also known as NCS1, is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord. It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells. NCS1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules. Recombinant human NCS1 fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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