NDUFA5 antibody (70R-13397)
Affinity purified Rabbit polyclonal NDUFA5 antibody
Overview
Overview
| Synonyms | Polyclonal NDUFA5 antibody, Anti-NDUFA5 antibody, B13 antibody, NDUFA 5 antibody, NDUFA5 antibody, DKFZp781K1356 antibody, FLJ12147 antibody, CI-13kB antibody, UQOR13 antibody, NUFM antibody, NDUFA 5, NADH-ubiquinone oxidoreductase 13 kDa-B subunit antibody, NDUFA-5, NDUFA5, NDUFA-5 antibody, type I dehydrogenase antibody, CI-13KD-B antibody |
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| Cross Reactivity | Human, Mouse |
| Applications | IHC-P, WB |
Images
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | NDUFA5 antibody was purified by antigen-affinity chromatography |
| Molecular Weight | 13 kDa |
| Form & Buffer | Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative. |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | IHC-P: 1:100-1:500, WB: 1:500-1:3000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. |
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