NDUFA5 antibody (70R-13397)

Affinity purified Rabbit polyclonal NDUFA5 antibody

Synonyms Polyclonal NDUFA5 antibody, Anti-NDUFA5 antibody, B13 antibody, NDUFA 5 antibody, NDUFA5 antibody, DKFZp781K1356 antibody, FLJ12147 antibody, CI-13kB antibody, UQOR13 antibody, NUFM antibody, NDUFA 5, NADH-ubiquinone oxidoreductase 13 kDa-B subunit antibody, NDUFA-5, NDUFA5, NDUFA-5 antibody, type I dehydrogenase antibody, CI-13KD-B antibody
Cross Reactivity Human, Mouse
Applications IHC-P, WB

Images

Western blot analysis using NDUFA5 antibody (70R-13397)

Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 15 % SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification NDUFA5 antibody was purified by antigen-affinity chromatography
Molecular Weight 13 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using NDUFA5 antibody (70R-13397)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 15 % SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using NDUFA5 antibody (70R-13397)</font></div>     | Immunohistochemical staining of paraffin-embedded Cal27 xenograft using NDUFA5 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using NDUFA5 antibody (70R-13397)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 15 % SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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