Nestin antibody (70R-10645)
Affinity purified Chicken polyclonal Nestin antibody
Overview
Overview
| Synonyms | Polyclonal Nestin antibody, Anti-Nestin antibody |
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| Applications | ICC, IHC, WB |
| Immunogen | Nestin antibody was raised in Chicken using a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate corresponding to the Nestin gene product as the immunogen. |
Images
Immunofluorescent staining of Nestin antibody (70R-10645)
A tissue section through an e13 mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows visualization of nuclei.
Specifications
| Host | Chicken |
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| Method of Purification | Nestin antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative. |
| Concentration | 100 ug/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:10000-1:20000; IHC: 1:10000-1:20000; WB: 1:20000-1:50000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | Nestin is an intermediate filament protein that is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon differentiation, Nestin is replaced by cell type-specific intermediate filaments, (e.g., neurofilaments and glial fibrillary acidic protein (GFAP)). Nestin expression is re-induced after CNS injury and during regeneration of injured muscle tissue. |
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