Nestin antibody (70R-10645)

Affinity purified Chicken polyclonal Nestin antibody

Synonyms Polyclonal Nestin antibody, Anti-Nestin antibody
Applications ICC, IHC, WB
Immunogen Nestin antibody was raised in Chicken using a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate corresponding to the Nestin gene product as the immunogen.

Images

Immunofluorescent staining of Nestin antibody (70R-10645)

A tissue section through an e13 mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows visualization of nuclei.

Specifications

Host Chicken
Method of Purification Nestin antibody was purified by affinity chromatography.
Form & Buffer Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative.
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 1:10000-1:20000; IHC: 1:10000-1:20000; WB: 1:20000-1:50000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light.

General Information

Biological Significance Nestin is an intermediate filament protein that is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon differentiation, Nestin is replaced by cell type-specific intermediate filaments, (e.g., neurofilaments and glial fibrillary acidic protein (GFAP)). Nestin expression is re-induced after CNS injury and during regeneration of injured muscle tissue.

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Images

  • Immunofluorescent staining of Nestin antibody (70R-10645) | A tissue section through an e13 mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows visualization of nuclei.

Availability: In stock

Price: €502.25
Size: 1 ml
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