NeuN antibody (70R-10629)

Affinity purified Chicken polyclonal NeuN antibody

Synonyms Polyclonal NeuN antibody, Anti-NeuN antibody, Fox 3 antibody, Fox-3 antibody, Neu-N antibody, Neu N antibody
Applications ICC, IHC
Immunogen NeuN antibody was raised in Chicken using a synthetic peptide (Human NP_001076044 and Mouse NP_001020102) / keyhole limpet hemocyanin (KLH) conjugate corresponding to the Fox3 (NeuN) gene product as the immunogen.

Images

Immunofluorescent staining of NeuN antibody (70R-10629)

CA1 region of a transgenic mouse expressing the eGFP gene product under control of the Thy-1 promoter. Green is Egfp autofluorescence; Red is Cy-3 secondary antibody labeling chicken anti-Neu-N (Fox-3) positive neuronal nuclei. Picture courtesy of Dr. Felix Eckenstein, University of Vermont.

Specifications

Host Chicken
Method of Purification NeuN antibody was purified by affinity chromatography.
Form & Buffer Supplied in liquid form in PBS buffer, pH 7.2, with 50% (v/v) glycerol and 0.02% (w/v) sodium azide.
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 1:2,000 - 1:5,000; IHC: 11:2,000 - 1:5,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light.

General Information

Biological Significance The vast majority of neurons are strongly NeuN positive, and NeuN immunoreactivity has been widely used to identify neurons is tissue culture and in sections and to measure the neuron/glial ratio in brain regions. NeuN immunoreactivity becomes obvious as neurons mature, typically after they have downregulated expression of Doublecortin, a marker seen in the earliest stages of neuronal development.

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Images

  • Immunofluorescent staining of NeuN antibody (70R-10629) | CA1 region of a transgenic mouse expressing the eGFP gene product under control of the Thy-1 promoter. Green is Egfp autofluorescence; Red is Cy-3 secondary antibody labeling chicken anti-Neu-N (Fox-3) positive neuronal nuclei. Picture courtesy of Dr. Felix Eckenstein, University of Vermont.

Availability: In stock

Price: €502.25
Size: 1 ml
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