NeuN antibody (70R-10629)
Affinity purified Chicken polyclonal NeuN antibody
Overview
Overview
| Synonyms | Polyclonal NeuN antibody, Anti-NeuN antibody, Fox 3 antibody, Fox-3 antibody, Neu-N antibody, Neu N antibody |
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| Applications | ICC, IHC |
| Immunogen | NeuN antibody was raised in Chicken using a synthetic peptide (Human NP_001076044 and Mouse NP_001020102) / keyhole limpet hemocyanin (KLH) conjugate corresponding to the Fox3 (NeuN) gene product as the immunogen. |
Images
Immunofluorescent staining of NeuN antibody (70R-10629)
CA1 region of a transgenic mouse expressing the eGFP gene product under control of the Thy-1 promoter. Green is Egfp autofluorescence; Red is Cy-3 secondary antibody labeling chicken anti-Neu-N (Fox-3) positive neuronal nuclei. Picture courtesy of Dr. Felix Eckenstein, University of Vermont.
Specifications
| Host | Chicken |
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| Method of Purification | NeuN antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in PBS buffer, pH 7.2, with 50% (v/v) glycerol and 0.02% (w/v) sodium azide. |
| Concentration | 100 ug/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:2,000 - 1:5,000; IHC: 11:2,000 - 1:5,000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | The vast majority of neurons are strongly NeuN positive, and NeuN immunoreactivity has been widely used to identify neurons is tissue culture and in sections and to measure the neuron/glial ratio in brain regions. NeuN immunoreactivity becomes obvious as neurons mature, typically after they have downregulated expression of Doublecortin, a marker seen in the earliest stages of neuronal development. |
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