Recognizes endogenous levels of NF-kappaB p65 protein
Cross Reactivity
Mouse, Rat, Bovine, Pig
Applications
ICC, IF, IHC, WB
Immunogen
NF kappaB p65 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p65 as the immunogen
Western blot analysis using NF kappaB p65 antibody (70R-50324)
Western blot analysis of NF-kappaB p65 expression in HepG2 (A); MCF7 (B); Raw264.7 (C); rat liver (D) whole cell lysates.
NF kappaB p65 antibody
Western blot analysis using NF kappaB p65 antibody (70R-50324)
Western blot analysis of NF-kappaB p65 expression in HepG2 (A); MCF7 (B); Raw264.7 (C); rat liver (D) whole cell lysates.
Immunohistochemical analysis using NF kappaB p65 antibody (70R-50324)
Immunohistochemical analysis of NF-kappaB p65 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis using NF kappaB p65 antibody (70R-50324)
Immunofluorescent analysis of NF-kappaB p65 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
NF kappaB p65 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
The activation of NF-kB is thought to be part of a stress response as it is activated by a variety of stimuli that include growth factors, cytokines, lymphokines, UV, pharmacological agents, and stress. In its inactive form, NF-kB is sequestered in the cytoplasm, bound by members of the IkB family of inhibitor proteins, which include IkBa, IkBb, IkBg, and IkBe.